Ipriflavone이 골 세포주(MC3T3-El cell line)의 Collagen합성에 미치는 영향
EFFECTS OF IPRIFLAVONE ON COLLAGEN SYNTHESIS OF OSTEOBLAST-LIKE CELLS(MC3T3-El CELL LINE)
소속 상세정보
양윤석/Yun-Seok Yang
KMID : 0360119970190040395
Abstract
Ipriflavone(IP)은 골흡수 억제효과와 골형성 촉진효과를 지니는 약물로 보고되어 왔다.
이러한 IP의 특성 때문에 골의 치유를 촉진시키기 위한 약물로서 구강외과 영역에서 쓰일
수도 있으리라 생각되었다.
이에 저자는 그 동안 보고되어온 IP의 골 형성 촉진효과가 실제로 나타나는지를 확인하고
또한 어떤 농도에서 나타나는지를 알아보기 위해 IP를 서로 다른 농도로 하여 골세포주
(MC3T3-El cell line)의 배지에 넣은 후, 골 형성의 지표로 쓰일 수 있는 collagen합성정도
를 보고자 하였으며 이 자료를 앞으로의 in vivo 동물실험 연구의 기초자료로 사용코자 하
였다.
본 연구에서 IP의 골형성 촉진효과를 collagen 합성정도의 측정을 통해 확인하였고, 특히
IP이 10-7M농도일 때 현저한 collagen합성의 증가를 관찰하였으며 앞으로의
동물실험등을 통해 구강외과 영역에서의 사용가능성에 대해 좀더 연구해 보고자 한다.
#초록#
Collagen, the most abundant protein in connective tissue, represents 90% of the
organic matrix of bone.
The collagen synthesis has been known as a good marker for the bone formation.
Therefore, 1 used the degree of collagen synthesis as a marker for the interpretation of
the effects of Ipriflavone(If) on the osteoblast-like cells.
In this study, 1 observed that IP stimulate the collagen systhesis of osteoblast-like
cells. This confirms previous in vitro observations on If's stimulation of collagen
systhesis by human otosclerotic auditory ossicel samples. This study showed that the
concentration which stimulate the collagen synthesis of osteoblast-like cells was 10-7M.
We also observed that the higher concentration (10-6M,10-5 M) inhibited the collagen
synthesis of osteoblast-like cells.
These date are not coincident with previous study(Sziklai et at . 1985) which showed
that the concentration of IP which stimulated the osteoblasts-like cells was 10-sM, but
coincident with the study(Ribari et at'1987) in the that 10-sM reduced the collagenous
protein synthesis.
These differences may be due to differences in culture conditions, cell species, and
durration of the exposure of the cells to IP.
PGE2 that is secreted by osteoblasts is currently the effective stimulator
of bone resorption and enhances the resorptive activity of osteoclasts. It also play a role
in collagen synthesis and high concentrations and increase.
Ribari et al reported that IP 10-5M decreased the collagen synthesis but
increased collagen synthesis inhibited by low concentration of PGE2.
Therefore, I think that the different concentration of PGE2 which was
secreted by osteoblast-like cells may be also a reason for the different results.
In conclusion, this study suggests that IP stimulated collagen synthesis of
osteoblast-like cells in 10-7M concentration.
Furthermore, IP may be used as a agent that facilitate bone healing in oral
maxillofacial surgery.
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